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Intracérébroventriculaire et intravasculaire Injection de particules virales et fluorescentes microbilles dans le cerveau néonatal
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain
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Intracérébroventriculaire et intravasculaire Injection de particules virales et fluorescentes microbilles dans le cerveau néonatal

18,905 Views

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05:51 min

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July 24, 2016

DOI:

10.3791/54164-v

DOI:
10.3791/54164-v

•

05:51 min
July 24, 2016

•

8 Views
, , , , ,
1Department of Regenerative & Infectious Pathology,Hamamatsu University School of Medicine, 2First Department of Medicine,Hamamatsu University School of Medicine, 3Faculty of Health Science,Tokoha University

Summary

Automatically generated

Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.

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IntracerebroventricularIntravascularViral ParticlesFluorescent MicrobeadsNeonatal BrainMurine CytomegalovirusLateral VentricleSuperficial Temporal VeinNeurovirologyAcute Infection PhaseVirus DistributionMicroscopyInjectionAnesthesiaNeonatal Mouse

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Cite this Article

Kawasaki, H., Kosugi, I., Sakao-Suzuki, M., Meguro, S., Tsutsui, Y., Iwashita, T. Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain. J. Vis. Exp. (113), e54164, doi:10.3791/54164 (2016).

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