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隔离和脂肪干细胞分化的猪从皮下脂肪组织
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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隔离和脂肪干细胞分化的猪从皮下脂肪组织

28,643 Views

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09:20 min

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March 31, 2016

DOI:

10.3791/53886-v

DOI:
10.3791/53886-v

•

09:20 min
March 31, 2016

•

13 Views
, , , , , ,
1Institute of Biotechnology,National Taiwan University, 2Department of Animal Science and Technology,National Taiwan University, 3Department of Surgery,National Taiwan University Hospital and College of Medicine

Summary

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This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

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Cite this Article

Chen, Y., Liu, H., Chang, Y., Cheng, Y., Mersmann, H. J., Kuo, W., Ding, S. Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues. J. Vis. Exp. (109), e53886, doi:10.3791/53886 (2016).

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