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الاستفادة المشتركة منهجيات لتحديد دور البلازما غشاء تسليم خلال أكسون التشعب والعصبية التشكل
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Capitoli
- 00:05Titolo
- 00:48SNARE Complex Formation Assay
- 03:12Imaging Exocytic Events via TIRF Microscopy
- 08:08Differential Interference Contrast Timelapse Microscopy of Axon Branching
- 10:10Toxin Manipulations and Fixed Cell Immunofluorescence
- 11:13Results: SNARE-mediated Exocytosis in Netrin-dependent Axon Branching
- 12:58Conclusion
Light microscopy techniques coupled with biochemical assays elucidate the involvement of SNARE-mediated exocytosis in netrin-dependent axon branching. This combination of techniques permits identification of molecular mechanisms controlling axon branching and cell shape change.
Tags
Combined MethodologiesPlasma Membrane DeliveryAxon BranchingNeuronal MorphogenesisQuantifiable ObservationsTemporal And Spatial OccurrenceExocytic Vesicle FusionNeuronal DevelopmentLive Cell ImagingHigh Spatial ResolutionHigh Temporal ResolutionBiochemical ApproachesPopulation-based InformationCulturing And Simulating Cortical NeuronsNetrin-1ControlHomogenization BufferProtein ConcentrationsTriton X-100Triturate MixtureSolubilize ProteinsCentrifuge
