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Sex Forskelle i Mus Hippocampale Astrocytter efter<em> In-vitro</em> Iskæmi
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Capitoli
- 00:05Titolo
- 00:42Hippocampal Dissection and Plating
- 03:32Astrocyte Culture
- 05:34Oxygen and Glucose Deprivation and Reoxygenation (OGD/REOX)
- 06:21Results: Increased Protein Expression of HIF1α and Astrocytic Markers Following OGD/REOX
- 07:46Conclusion
Astrocytter er en af de vigtigste centrale aktører i centralnervesystemet (CNS). Her bliver vi rapporterer en praktisk metode til kønsbestemt hippocampus astrocyt kultur protokol for at studere mekanismerne bag astrocyt funktion i mandlige og kvindelige nyfødte hvalpe efter in vitro-iskæmi.
Tags
Sex DifferencesMouse Hippocampal AstrocytesIn-vitro IschemiaEnriched CulturesOxygen And Glucose DeprivationReoxygenationHypoxic IschemiaMechanistic QuestionsTranslational QuestionsDeveloping Male And Female BrainsBrain DissectionNewborn Mouse PupPosterior To Anterior Midline IncisionCranium RemovalBrainstem RemovalDissecting DishHemispheres SeparationCerebral Hemispheres PeelingHippocampus RemovalMeninges And Fibroblasts Cleaning
