Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.
Kataoka, C., Kashiwada, S. Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs. J. Vis. Exp. (109), e53550, doi:10.3791/53550 (2016).