Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This Contenuto is Open Access.

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
 

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Article DOI: 10.3791/53464-v 06:47 min December 12th, 2015
December 12th, 2015

Capitoli

Riepilogo

Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.

Tags

Extracellular Acid Production Glycolytic Rate Respiration Mitochondrial Oxygen Consumption Lactate CO2 C2C12 Myoblasts Extracellular Acidification
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter