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Biomimetische Materialien zu den Bakterien-Wirt-Interaktionen Charakterisieren
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Biomimetic Materials to Characterize Bacteria-host Interactions
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Biomimetische Materialien zu den Bakterien-Wirt-Interaktionen Charakterisieren

8,955 Views

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12:22 min

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November 16, 2015

DOI:

10.3791/53400-v

DOI:
10.3791/53400-v

•

12:22 min
November 16, 2015

•

9 Views
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1Institute of Microbiology and Infection, School of Biosciences,University of Birmingham

Summary

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Bacterial attachment to host cells is a key step during host colonization and infection. This protocol describes the generation of polymer-coupled recombinant adhesins as biomimetic materials which allow analysis of the contribution of individual adhesins to these processes, independent of other bacterial factors.

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Biomimetic MaterialsBacteria-host InteractionsBacterial AdhesinsRecombinant ProteinsPolystyrene BeadsProtein ImmobilizationBacterial ColonizationHost Cell ReceptorsBacterial CytotoxicityBacterial Infection

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Cite this Article

Stones, D. H., Al-Saedi, F., Vaz, D., Perez-Soto, N., Krachler, A. M. Biomimetic Materials to Characterize Bacteria-host Interactions. J. Vis. Exp. (105), e53400, doi:10.3791/53400 (2015).

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