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Hochdurchsatz-<em> Danio rerio</em> Energieumsatz-Assay
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Biologia
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JoVE Journal Biologia
High Throughput Danio Rerio Energy Expenditure Assay
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Hochdurchsatz-<em> Danio rerio</em> Energieumsatz-Assay

DOI:
10.3791/53297-v

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08:35 min

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January 27, 2016

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,
1Department of Molecular Physiology and Biophysics,Vanderbilt University Medical Center, 2School of Animal and Comparative Biomedical Sciences,University of Arizona

Capitoli

  • 00:05Titolo
  • 01:01Zebrafish Breeding and Maintenance
  • 02:33Energy Expenditure Assay
  • 06:10Results: Energy Expenditure Assay Results
  • 07:28Conclusion

Summary

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Zebrafish are an important model organism for the study of energy homeostasis. By utilizing a NADH2 sensitive redox indicator, alamar Blue, we have developed an assay that measures the metabolic rate of zebrafish larvae in a 96 well plate format and can be applied to drug or gene discovery.

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Keywords: High ThroughputDanio RerioEnergy ExpenditureAssayResazurinFluorometricNADH2Metabolic RateObesityDrug-drug InteractionDrug-gene InteractionOxygen ConsumptionEmbryonic FishZebrafishE3 MediumMethylene BlueFlame PolishPipetteFertilized EggsDead EmbryosAssay SolutionFilter Sterilize

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