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方法调查小RNA和核糖体占用的调控作用<em>恶性疟原虫</em
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
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方法调查小RNA和核糖体占用的调控作用<em>恶性疟原虫</em

8,742 Views

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10:22 min

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December 04, 2015

DOI:

10.3791/53214-v

DOI:
10.3791/53214-v

•

10:22 min
December 04, 2015

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1 Views
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1Department of Molecular Genetics and Microbiology,Duke University School of Medicine, 2Center for Genomic and Computational Biology,Duke University School of Medicine, 3Department of Cell Biology,Duke University School of Medicine

Summary

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Human microRNAs translocate from host erythrocytes to Plasmodium falciparum parasites. Here, the techniques used to transfect synthetic microRNAs into host erythrocytes and isolate all RNAs from P. falciparum are described. In addition, this paper will detail a method of polysome isolation in P. falciparum to determine the ribosomal occupancy and translational potential of parasite transcripts.

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Cite this Article

LaMonte, G., Walzer, K. A., Lacsina, J., Nicchitta, C., Chi, J. Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum. J. Vis. Exp. (106), e53214, doi:10.3791/53214 (2015).

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