/
/
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
9,334 Views
•
09:40 min
•
September 30, 2015
DOI:
Summary
Automatically generated
This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.
Read Article
Cite this Article
Shirazi Fard, S., Blixt, M., Hallböök, F. Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments. J. Vis. Exp. (103), e53202, doi:10.3791/53202 (2015).
Copy