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Analyse von Zebrafisch-Larven Skelettmuskel Integrität mit Evans Blue Dye
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Analyse von Zebrafisch-Larven Skelettmuskel Integrität mit Evans Blue Dye
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07:34 min
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November 30, 2015
DOI:
1Program in Genetics & Genome Biology,The Hospital for Sick Children, 2Department of Molecular Genetics,The University of Toronto, 3Program in Genomics of Differentiation,Eunice Kennedy Shriver National Institute of Child Health and Human Development, 4Departments of Pediatrics and Neurology,University of Michigan
Summary
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In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.
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Smith, S. J., Horstick, E. J., Davidson, A. E., Dowling, J. Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye. J. Vis. Exp. (105), e53183, doi:10.3791/53183 (2015).
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