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Protokoll zur dreidimensionalen konfokalen morphometrische Analyse der Astrozyten
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Neuroscienze
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JoVE Journal Neuroscienze
Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes
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Protokoll zur dreidimensionalen konfokalen morphometrische Analyse der Astrozyten

DOI:
10.3791/53113-v

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09:13 min

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December 11, 2015

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, , , ,
1Department of Clinical and Experimental Medicine,Linköping University, 2Neurophysiology Research Center,Shahed University, 3Cellular and Molecular Research Center,Iran University of Medical Sciences, 4School of Advanced Technologies in Medicine,Iran University of Medical Sciences

Capitoli

  • 00:05Titolo
  • 00:46Immunohistochemistry of Rat Brain Slices
  • 02:38Confocal Microscopy
  • 04:51Data Analysis of 2D and 3D Confocal Images
  • 07:17Results: Qualitative and Quantitative Analysis of Astrogliosis
  • 08:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

Astrocytes in the CNS change their functional and structural properties in response to harmful stimuli. This report presents a protocol for assessment of three-dimensional astrocyte morphology in diseased conditions or after therapeutic interventions.

Tags

Keywords: 3D Confocal MicroscopyAstrocyte MorphologyAstrocyte TerritoryAstrocyte VolumeAstrocyte DensityReactive AstrogliosisAmyloid BetaImage AnalysisVolocity Software

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