This content is Free Access.
ושאריות או זיהוי של Interpositional תלות המציין מבני / צמודות תפקודית: אופטימיזציה של חלבונים סינטטיים
7,078 Views
•
07:08 min
•
July 14, 2015
DOI:
Summary
Automatically generated
Synthetic protein sequences based on consensus motifs typically ignore co-evolving residues, that imply interpositional dependencies (IPDs). IPDs can be essential to activity, and designs that disregard them may result in suboptimal results. This protocol uses StickWRLD to identify IPDs and help inform rational protein design, resulting in more efficient results.
Read Article
Cite this Article
Rumpf, R. W., Ray, W. C. Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues. J. Vis. Exp. (101), e52878, doi:10.3791/52878 (2015).
Copy