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的自然杀伤细胞免疫突触上的玻璃支承平面脂双层超分辨率成像
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
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的自然杀伤细胞免疫突触上的玻璃支承平面脂双层超分辨率成像

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09:56 min

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February 11, 2015

DOI:

10.3791/52502-v

DOI:
10.3791/52502-v

•

09:56 min
February 11, 2015

•

5 Views
, , , ,
1Center for Human Immunobiology,Texas Children’s Hospital, 2Department of Pediatrics,Baylor College of Medicine, 3Department of Pathology and Immunology,Baylor College of Medicine

Summary

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We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

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Super-resolution ImagingNatural Killer CellImmunological SynapseGlass-supported Planar Lipid BilayerSTED MicroscopyPerforinFilamentous ActinNK Cell Activation Receptor CD16

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Cite this Article

Zheng, P., Bertolet, G., Chen, Y., Huang, S., Liu, D. Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer. J. Vis. Exp. (96), e52502, doi:10.3791/52502 (2015).

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