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إنشاء الإنسان الظهارية Enteroids وColonoids من الأنسجة كامل وخزعة
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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إنشاء الإنسان الظهارية Enteroids وColonoids من الأنسجة كامل وخزعة

33,904 Views

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06:33 min

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March 06, 2015

DOI:

10.3791/52483-v

DOI:
10.3791/52483-v

•

06:33 min
March 06, 2015

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13 Views
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1Department of Pediatric General and Thoracic Surgery,Cincinnati Children’s Hospital Medical Center, 2Department of Medicine, Section of Gastroenterology and Hepatology,Baylor College of Medicine

Summary

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We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

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Cite this Article

Mahe, M. M., Sundaram, N., Watson, C. L., Shroyer, N. F., Helmrath, M. A. Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy. J. Vis. Exp. (97), e52483, doi:10.3791/52483 (2015).

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