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原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类
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Neuroscienze
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JoVE Journal Neuroscienze
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
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原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

13,635 Views

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08:45 min

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February 19, 2015

DOI:

10.3791/52475-v

DOI:
10.3791/52475-v

•

08:45 min
February 19, 2015

•

14 Views
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1Division of Brain Sciences, Department of Medicine,Imperial College London, 2Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Internal Medicine, Endocrinology,Yale University School of Medicine

Summary

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

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Primary Mesencephalic Dopaminergic NeuronsEmbryonic Rodent BrainsParkinson’s DiseaseNeurodegenerative DisorderCell CultureMidbrain Dopaminergic NeuronsE12.5 MouseE14.5 RatNeuronal MorphologyPhysiological Characteristics

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Weinert, M., Selvakumar, T., Tierney, T. S., Alavian, K. N. Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains. J. Vis. Exp. (96), e52475, doi:10.3791/52475 (2015).

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