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Synthese van Indoxyl-glycosiden voor detectie van Glycosidase Activiteiten
JoVE Journal
Chimica
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JoVE Journal Chimica
Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Synthese van Indoxyl-glycosiden voor detectie van Glycosidase Activiteiten

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09:10 min

May 27, 2015

DOI:

09:10 min
May 27, 2015

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Summary

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Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good yields.

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