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Valutazione dell'impatto di esposizione ripetuta di organotipica 3D bronchiali e nasali coltura tissutale modelli di Whole fumo di sigaretta
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Valutazione dell'impatto di esposizione ripetuta di organotipica 3D bronchiali e nasali coltura tissutale modelli di Whole fumo di sigaretta
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February 12, 2015
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The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
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Kuehn, D., Majeed, S., Guedj, E., Dulize, R., Baumer, K., Iskandar, A., Boue, S., Martin, F., Kostadinova, R., Mathis, C., Ivanov, N. V., Frentzel, S., Hoeng, J., Peitsch, M. C. Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke. J. Vis. Exp. (96), e52325, doi:10.3791/52325 (2015).
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