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Meten Eiwit Stabiliteit in Living zebravis embryo's met behulp van fluorescentie Decay Na Photoconversion (FDAP)
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
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Meten Eiwit Stabiliteit in Living zebravis embryo's met behulp van fluorescentie Decay Na Photoconversion (FDAP)

11,075 Views

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09:45 min

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January 28, 2015

DOI:

10.3791/52266-v

DOI:
10.3791/52266-v

•

09:45 min
January 28, 2015

•

4 Views
, , ,
1Department of Molecular and Cellular Biology,Harvard University, 2Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Summary

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Eiwitniveaus in cellen en weefsels vaak zijn gereguleerd door het evenwicht van eiwitproductie en klaring. Met behulp van fluorescentie Decay Na Photoconversion (FDAP), kan de klaringskinetiek van eiwitten experimenteel worden gemeten in vivo.

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Cite this Article

Rogers, K. W., Bläßle, A., Schier, A. F., Müller, P. Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP). J. Vis. Exp. (95), e52266, doi:10.3791/52266 (2015).

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