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对专为递送治疗因素的神经保护策略成人干细胞高通量表征
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
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对专为递送治疗因素的神经保护策略成人干细胞高通量表征

DOI:
10.3791/52242-v

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09:19 min

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January 04, 2015

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, , , , , ,
1Department of Chemical and Biological Engineering,Iowa State University, 2Department of Genetics, Development and Cell Biology,Iowa State University, 3Biology Program,Iowa State University

Capitoli

  • 00:05Titolo
  • 01:45Substrate Preparation and Cell Plating
  • 03:35Time Lapse Imaging
  • 05:01Ki67 Cell Proliferation, Automated Imaging and Multi-wavelength Scoring Analysis
  • 07:00Results: Characterization of Engineered MSCs
  • 08:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

This study describes an experimental platform to rapidly characterize engineered stem cells and their behaviors before their application in long-term in vivo transplant studies for nervous system rescue and repair.

Tags

Mesenchymal Stem CellsGenetic ModificationBrain-derived Neurotrophic FactorGlial Cell-derived Neurotrophic FactorHigh Content ScreeningCell AdhesionCell ProliferationCell MigrationCell ViabilityNeuroprotective Strategies

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