/

/

Субклонирование Плюс погружения (SPI) - Роман Recombineering способом быстрого строительства генного таргетинга векторы

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Please note that all translations are automatically generated. Click here for the English version.

Субклонирование Плюс погружения (SPI) - Роман Recombineering способом быстрого строительства генного таргетинга векторы

16,105 Views

•

09:02 min

•

January 08, 2015

DOI:

10.3791/52155-v

DOI:

10.3791/52155-v

•

09:02 min

January 08, 2015

•

2 Views

Thimma R. Reddy, Emma J. Kelsall, Léna M.S. Fevat, Sarah E. Munson*³, Shaun M. Cowley*¹

¹Department of Biochemistry,University of Leicester, ²Center for Fisheries, Environment and Aquaculture Sciences, ³ES Cell Facility, Centre for Core Biotechnology Services,University of Leicester

Tags

Subcloning Recombineering Gene Targeting Vector Homologous Recombination Bacterial Artificial Chromosome Conditional Gene Knockout Knock-in Vector BAC Reporter Vector Selection Marker Multiplex Approach Plasmid Gap Repair

Read Article