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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
 

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

Article DOI: 10.3791/52084-v • 10:02 min • October 21st, 2014
October 21st, 2014 •
1, 1
1Department of Microbiology and Immunology, Albert Einstein College of Medicine

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We describe how to visualize macrophage-C. neoformans (Cn) interactions in real time, with specific emphasis on the process of non-lytic exocytosis using digital light microscopy. Using this technique individually infected macrophages can be studied to ascertain various aspects of this phenomenon.

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Non-lytic Exocytosis Cryptococcus Neoformans Macrophages Digital Light Microscopy Infection Yeast Cells Extracellular Space Mechanism Viability Time-lapsed Microscopy Heating Chamber CO2 Atmosphere Cell-culture Incubator Live Digital Microscopy Dynamic Interactions Host-pathogen Relationship Fungal Infections
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