/
/
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Capitoli
- 00:05Titolo
- 01:09General Preparations
- 02:41Dissection and Preparation of Tissue Slices
- 04:57Tissue Microdissection and Cell Dissociation, Sorting, and Plating
- 06:46Results of Isolating and Culturing NEPs
- 07:43Conclusion
Nestin-expressing progenitors are a newly identified population of neuronal progenitors in the developing cerebellum. Using the microdissection technique presented here in combination with fluorescent-activated cell sorting, this cell population can be purified with no contamination from other cerebellar regions and can be cultured for further studies.
