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对基因差异表达的RNA序列中电穿孔鸡胚脊髓分析
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Neuroscienze
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JoVE Journal Neuroscienze
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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对基因差异表达的RNA序列中电穿孔鸡胚脊髓分析

14,379 Views

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11:13 min

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November 01, 2014

DOI:

10.3791/51951-v

DOI:
10.3791/51951-v

•

11:13 min
November 01, 2014

•

2 Views
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1Department of Cell and Developmental Biology,Universidade de São Paulo

Summary

Automatically generated

This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.

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RNA-SeqDifferential Gene ExpressionChick Embryonic Spinal CordIn Ovo ElectroporationNeural DevelopmentTranscriptome AnalysisGene FunctionBioinformaticsGalaxy Public ServerHigh-quality RNA SamplesOverexpression

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Cite this Article

Vieceli, F. M., Yan, C. I. RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord. J. Vis. Exp. (93), e51951, doi:10.3791/51951 (2014).

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