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एक उपन्यास और सुलभ दृष्टिकोण: स्ट्रक्चरल HEK 293F निलंबन कोशिकाओं में जीवविज्ञान के लिए पुनः संयोजक प्रोटीन अभिव्यक्ति
Capitoli
- 00:05Titolo
- 01:48Starter Cultures
- 03:43Large-scale Culture/Transfection in 1 L Roller Bottles
- 05:16Protein-complex Purification from Whole Cell Extract
- 09:20Results: Expression and Purification of the HDAC1, SDS3, and Sin3A Ternary Complex
- 10:35Conclusion
The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.
