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13:14 min
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August 22, 2014
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In planta measurement of nutrient and toxicant fluxes is essential to the study of plant nutrition and toxicity. Here, we cover radiotracer protocols for influx and efflux determination in intact plant roots, using potassium (K+) and ammonia/ammonium (NH3/NH4+) fluxes as examples. Advantages and limitations of such techniques are discussed.
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Coskun, D., Britto, D. T., Hamam, A. M., Kronzucker, H. J. Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers. J. Vis. Exp. (90), e51877, doi:10.3791/51877 (2014).
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