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Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders
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JoVE Journal Neuroscienze
Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

DOI:
10.3791/51853-v

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08:33 min

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December 04, 2014

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, , , , , , , , ,
1Department of Psychiatry,Johns Hopkins University, 2Department of Psychiatry and Behavioral Sciences,Howard University, 3Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins University, 4Department of Psychiatry,Sheppard Pratt Hospital, 5Department of Psychiatry,Indiana University

Capitoli

  • 00:05Titolo
  • 01:49Laser-Capture Microdissection
  • 03:48Isolation of Total RNA and cDNA Synthesis
  • 05:50Real Time PCR Quantification
  • 07:14Reults: GSK-3β Expression in OE Neurons
  • 07:53Conclusion

Summary

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In this study, a novel platform to investigate intraneuronal molecular signatures of treatment response in bipolar disorder (BD) was developed and validated. Olfactory epithelium from BD patients was obtained through nasal biopsies. Then laser-capture microdissection was combined with Real Time RT PCR to investigate the molecular signature of lithium response in BD.

Tags

Olfactory NeuronsNasal BiopsyLaser-capture MicrodissectionBipolar DisorderLithium CarbonateWnt SignalingNeuropsychiatric DisordersOlfactory EpitheliumTreatment Response

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