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心房と心室から心筋細胞における管状膜ネットワークの解析
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
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心房と心室から心筋細胞における管状膜ネットワークの解析

20,292 Views

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10:30 min

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October 15, 2014

DOI:

10.3791/51823-v

DOI:
10.3791/51823-v

•

10:30 min
October 15, 2014

•

6 Views
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1Heart Research Center Goettingen, 2Clinic of Cardiology & Pulmonology,University Medical Center Goettingen, 3German Center for Cardiovascular Research (DZHK) partner site Goettingen, 4BioMET, Center for Biomedical Engineering & Technology,University of Maryland School of Medicine

Summary

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心筋細胞では、管状の膜構造は、細胞内のネットワークを形成している。私たちは、品質管理、最先端の蛍光顕微鏡用ii）の生細胞染色を含むマウスの心臓から心筋細胞のi）の単離のためのプロトコルを最適化について説明し、およびiii）直接画像解析は、コンポーネントの複雑さおよび細胞内膜の可塑性を定量化するネットワーク。

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Wagner, E., Brandenburg, S., Kohl, T., Lehnart, S. E. Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles. J. Vis. Exp. (92), e51823, doi:10.3791/51823 (2014).

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