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ماركر الخلايا السطحية تنقية بوساطة من الجذع الوسطيات خلية من نموذج قابل للبرمجة ماوس
JoVE Journal
Biologia
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JoVE Journal Biologia
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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ماركر الخلايا السطحية تنقية بوساطة من الجذع الوسطيات خلية من نموذج قابل للبرمجة ماوس

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10:32 min

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September 06, 2014

DOI:

10.3791/51728-v

DOI:
10.3791/51728-v

•

10:32 min
September 06, 2014

•

3 Views
, , , ,
1Department of Anatomy and Developmental Biology,Monash University, 2Australian Regenerative Medicine Institute,Monash University

Summary

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Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.

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Nefzger, C. M., Alaei, S., Knaupp, A. S., Holmes, M. L., Polo, J. M. Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model. J. Vis. Exp. (91), e51728, doi:10.3791/51728 (2014).

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