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In vivo optogenetische Stimulation des Nager Central Nervous System
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Neuroscienze
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JoVE Journal Neuroscienze
In vivo Optogenetic Stimulation of the Rodent Central Nervous System
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In vivo optogenetische Stimulation des Nager Central Nervous System

DOI:
10.3791/51483-v

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09:37 min

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January 15, 2015

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, , , , ,
1Department of Psychiatry,University of Pittsburgh Medical Center, 2Department of Bioengineering,Stanford University, 3Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory,Massachusetts Institute of Technology, 4Department of Neurobiology and Behavior,Cornell University, 5Department of Psychiatry and Behavioral Sciences,Stanford University

Capitoli

  • 00:05Titolo
  • 01:46Non-contact Style Laser Coupling
  • 05:13In vivo Optogenetic Stimulation and Post In vivo Considerations
  • 08:00Results: Rodent Behavioral Results from Optogenetic Stimulation
  • 09:10Conclusion

Summary

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Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.

Tags

Keywords: OptogeneticsIn VivoCentral Nervous SystemNeural CircuitsCell-type SpecificitySpatial PrecisionTemporal ResolutionLight-sensitive Ion ChannelsOpsinsLaser StimulationTetheringBehavioral Testing

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