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高效的生产和纯化重组小鼠Kindlin-3从昆虫细胞生物物理研究
JoVE Journal
Biologia
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JoVE Journal Biologia
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
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高效的生产和纯化重组小鼠Kindlin-3从昆虫细胞生物物理研究

23,106 Views

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13:52 min

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March 19, 2014

DOI:

10.3791/51206-v

DOI:
10.3791/51206-v

•

13:52 min
March 19, 2014

•

4 Views
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1Division of Structural Biology, Wellcome Trust Centre for Human Genetics,University of Oxford

Summary

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Kindlins是基本的细胞粘附通过整合，但他们的研究已经阻碍了在细菌宿主中重组表达他们所遇到的困难。我们在这里描述它们高效的生产在杆状病毒感染的昆虫细胞的方法。

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Cite this Article

Yates, L. A., Gilbert, R. J. C. Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies. J. Vis. Exp. (85), e51206, doi:10.3791/51206 (2014).

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