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Çift Renkli Zaman-kapılı Uyarılmış Emisyon tükenmesi (STED) Nanoscopy tarafından İmmünoloji Synapse görselleştirme
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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Çift Renkli Zaman-kapılı Uyarılmış Emisyon tükenmesi (STED) Nanoscopy tarafından İmmünoloji Synapse görselleştirme

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10:00 min

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March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

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10:00 min
March 24, 2014

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1 Views
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1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Summary

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Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.

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Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

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