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La visualización de la sinapsis inmunológica mediante gated-Tiempo color dual Emisión Estimulada de Agotamiento (STED) Nanoscopia
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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La visualización de la sinapsis inmunológica mediante gated-Tiempo color dual Emisión Estimulada de Agotamiento (STED) Nanoscopia

77,743 Views

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10:00 min

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March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

•

10:00 min
March 24, 2014

•

1 Views
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1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Summary

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Aquí se ilustra el protocolo para obtener imágenes de dos colores STED nanoscopía la sinapsis inmune citotóxica de las células NK recapitulado sobre vidrio. Con este método se obtiene la resolución nm sub-100 de las proteínas de la sinapsis y el citoesqueleto.

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Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

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