Rivista
/
Immunologia e infezioni
/
Visualizzazione della immunologica Synapse da Dual Color Time-gated emissione stimolata Depletion (STED) Nanoscopia
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Please note that all translations are automatically generated. Click here for the English version.

Visualizzazione della immunologica Synapse da Dual Color Time-gated emissione stimolata Depletion (STED) Nanoscopia

77,743 Views

•

10:00 min

•

March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

•

10:00 min
March 24, 2014

•

1 Views
,
1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Summary

Automatically generated

Qui illustriamo il protocollo per l'imaging a due colori STED nanoscopia il citotossica sinapsi immunitaria delle cellule NK ricapitolato su vetro. Usando questo metodo si ottiene nm risoluzione sub-100 di proteine ​​sinaptiche e il citoscheletro.

Explore More Videos

Immunological SynapseNatural Killer CellsActin ReorganizationSuper-resolution ImagingStimulated Emission Depletion (STED) NanoscopyTime-gated STEDCytotoxic SynapseF-actin Architecture

Read Article

Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

Download .ris file

Copy

Share Video

.

Copy