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Ottimizzato colorazione negativa: un protocollo ad alta velocità per l'esame di piccole e asimmetrica Protein Structure da microscopia elettronica
JoVE Journal
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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
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Ottimizzato colorazione negativa: un protocollo ad alta velocità per l'esame di piccole e asimmetrica Protein Structure da microscopia elettronica

42,338 Views

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09:37 min

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August 15, 2014

DOI:

10.3791/51087-v

DOI:
10.3791/51087-v

•

09:37 min
August 15, 2014

•

15 Views
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1Lawrence Berkeley National Laboratory,The Molecular Foundry

Tags

Negative StainingElectron MicroscopyHigh-throughputProtein StructureSmall ProteinsAsymmetric ProteinsCryo-EMLipoproteinRouleauxElectron TomographyCholesteryl Ester Transfer Protein (CETP)

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Rames, M., Yu, Y., Ren, G. Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy. J. Vis. Exp. (90), e51087, doi:10.3791/51087 (2014).

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