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优化负染：通过电子显微镜检查小和不对称蛋白结构的高通量协议
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
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优化负染：通过电子显微镜检查小和不对称蛋白结构的高通量协议

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09:37 min

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August 15, 2014

DOI:

10.3791/51087-v

DOI:
10.3791/51087-v

•

09:37 min
August 15, 2014

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15 Views
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1Lawrence Berkeley National Laboratory,The Molecular Foundry

Tags

Negative StainingElectron MicroscopyHigh-throughputProtein StructureSmall ProteinsAsymmetric ProteinsCryo-EMLipoproteinRouleauxElectron TomographyCholesteryl Ester Transfer Protein (CETP)

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Rames, M., Yu, Y., Ren, G. Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy. J. Vis. Exp. (90), e51087, doi:10.3791/51087 (2014).

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