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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

JoVE Journal
Ambiente

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JoVE Journal Ambiente

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

42,338 Views

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09:37 min

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August 15, 2014

DOI:

10.3791/51087-v

DOI:

10.3791/51087-v

•

09:37 min

August 15, 2014

•

15 Views

Matthew Rames, Yadong Yu, Gang Ren

¹Lawrence Berkeley National Laboratory,The Molecular Foundry

Tags

Negative Staining Electron Microscopy High-throughput Protein Structure Small Proteins Asymmetric Proteins Cryo-EM Lipoprotein Rouleaux Electron Tomography Cholesteryl Ester Transfer Protein (CETP)

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