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Aktif Kaspaz 3 Algılama Tüm montaj immünofloresans tarafından Zebra balığı Embriyolar Apaptozun Analizi
JoVE Journal
Biologia
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JoVE Journal Biologia
Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
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Aktif Kaspaz 3 Algılama Tüm montaj immünofloresans tarafından Zebra balığı Embriyolar Apaptozun Analizi

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08:20 min

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December 20, 2013

DOI:

10.3791/51060-v

DOI:
10.3791/51060-v

•

08:20 min
December 20, 2013

•

18 Views
, , ,
1Department of Oncological Sciences,University of Utah

Summary

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Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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ZebrafishEmbryosApoptosisWhole-mount ImmunofluorescenceActivated Caspase 3TUNEL AssayIntrinsic ApoptosisExtrinsic ApoptosisCell TypesFixed EmbryosMutantsMorphantsOverexpressionChemicalsAcridine OrangeCost-effectiveReliable

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Cite this Article

Sorrells, S., Toruno, C., Stewart, R. A., Jette, C. Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3. J. Vis. Exp. (82), e51060, doi:10.3791/51060 (2013).

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