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Proteomiske Prøveforberedelse fra formalin Faste og Paraffin Embedded Tissue
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
DOI:

09:20 min

September 02, 2013

Capitoli

  • 00:05Titolo
  • 01:46Preparation of Tissue Slices for Microdissection and Sample Lysis
  • 03:20Sample Processing
  • 04:51Quantification of the Peptides
  • 05:38Fractionation of the Lys-C and Tryptic Peptides
  • 07:29Results: Peptide Quantification
  • 08:21Conclusion

Summary

Traduzione automatica

Archival formalin faste og paraffinindlejret (FFPE) kliniske prøver er værdifuldt materiale til undersøgelse af sygdomme. Her demonstrerer vi en prøveforberedelse workflow tillader indgående proteomisk analyse af mikrodissekeres FFPE væv.

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