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Visualisatie en analyse van mRNA moleculen met behulp van fluorescentie<em> In Situ</em> Hybridisatie in<em> Saccharomyces cerevisiae</em
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualisatie en analyse van mRNA moleculen met behulp van fluorescentie<em> In Situ</em> Hybridisatie in<em> Saccharomyces cerevisiae</em

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

•

07:00 min
June 14, 2013

•

3 Views
, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Summary

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Dit protocol beschrijft een experimentele procedure voor het uitvoeren Fluorescentie<em> In situ</em> Hybridisatie (FISH) voor het tellen van mRNA's in enkele cellen in single-molecule resolutie.

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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Cite this Article

McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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