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Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
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JoVE Journal Neuroscienze
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

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16:33 min

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February 18, 2013

DOI:

10.3791/50031-v

DOI:
10.3791/50031-v

•

16:33 min
February 18, 2013

•

17 Views
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1Department of Cellular and Physiological Sciences, Brain Research Centre,University of British Columbia

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Protein PalmitoylationAcyl-biotin Exchange (ABE) AssayImmunoprecipitationHippocampal NeuronsProtein TraffickingPost-translational ModificationLipid ModificationCysteineHydroxylamineBiotinWestern Blotting

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Brigidi, G. S., Bamji, S. X. Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE). J. Vis. Exp. (72), e50031, doi:10.3791/50031 (2013).

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