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13:28 min
February 15, 2012
DOI:
10.3791/3796-v
微生物生物膜一般由专门的细胞不同亚群构成。这些亚群的单细胞分析需要使用荧光记者。在这里,我们描述了一个协议,以可视化和监视几个subpopulationswithin<em> B枯草</em>生物膜,用荧光显微镜和流式细胞仪。
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Garcia-Betancur, J. C., Yepes, A., Schneider, J., Lopez, D. Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry. J. Vis. Exp. (60), e3796, doi:10.3791/3796 (2012).
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