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Bestemmelse Optimal cytotoksisk aktivitet for Human Her2neu Specifikke CD8 T-celler ved at sammenligne de Cr51 Udgivelse Assay til xCELLigence System
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Capitoli
- 00:05Titolo
- 01:16Generation of Short-term Human Antigen-specific CD8 T Cell Lines
- 02:55Preparation of Breast Cancer Cells
- 04:19Co-culture of Tumor with CD8 T Cells
- 05:45Chromium Release Assay (CRA)
- 07:21HER-2/neu p369-specific T Cell / SKBR3 Breast Cancer Cells Co-cultures
- 11:09Conclusion
Den chromfrigivelsesanalyse, en fælles assay til påvisning cytotoksisk T-celle-aktivitet, har adskillige begrænsninger. Brug antigenspecifikke CD8 T-celler og human brystcancer tumor linje, SKBR3, i nærværende artikel, var en impedans tilgang undersøgt for evnen til at detektere celledrab.
Tags
Cytotoxic ActivityCD8 T CellsCr51 Release AssayXCELLigence SystemT Cell Receptor (TCR)Antigen-specific CD8 T CellsELIspotFlow CytometryCytotoxicity AssayChromium Release Assay (CRA)Gamma RadiationReproducibilityCaspase ReleaseBLT Esterase ActivityCD107 ExpressionImpedance-based AssayRoche XCelligence System
