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神经元形态的研究方法：<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

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神经元形态的研究方法：<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔

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10:12 min

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May 18, 2012

DOI:

DOI:

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10:12 min

May 18, 2012

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2 Views

Sofia B. Lizarraga^1,2,3, Kathryn R. Coser^1,2, Mark Sabbagh^1,2, Eric M. Morrow^1,2,3

¹Department of Molecular, Cellular Biology and Biochemistry,Brown University , ²Institute for Brain Science,Brown University , ³Department of Psychiatry and Human Behavior, Warren Alpert School of Medicine,Brown University

Summary

我们描述涉及的方法进行快速评估的功能基因在大脑皮层的发展，<em>体外</em>电质粒共表达抑制核糖核酸（RNA干扰）和绿色荧光蛋白在小鼠胚胎皮层。该协议是适合研究神经发育的各个方面，如神经，神经细胞迁移和神经元的形态，包括树突和轴突生长。

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