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Post-natal murin Dokular Co-kültürler Myelinating zenginleştirilmiş oligodendrosit Kültürleri ve oligodendrosit / Nöron türetilmesi
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Neuroscienze
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JoVE Journal Neuroscienze
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
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Post-natal murin Dokular Co-kültürler Myelinating zenginleştirilmiş oligodendrosit Kültürleri ve oligodendrosit / Nöron türetilmesi

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18:17 min

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August 21, 2011

DOI:

10.3791/3324-v

DOI:
10.3791/3324-v

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18:17 min
August 21, 2011

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4 Views
, , ,
1Regenerative Medicine Program,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3Department of Pharmacological Sciences,Stony Brook University, 4Department of Medicine,University of Ottawa

Summary

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Bu makale, olgun oligodendrosit (EKK) üretmek için ayırt primer kültür fare oligodendrosit öncü hücreler (OPC) zenginleştirilmiş nüfus, elde etmek için yöntemler anlatılmaktadır. Buna ek olarak, bu rapor üretmek için teknikler açıklar fare fare dorsal kök ganglion nöronlar (DRGNs) neurite yatağa fare OPC ekim ortak kültürler myelinating.

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Cite this Article

O'Meara, R. W., Ryan, S. D., Colognato, H., Kothary, R. Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues. J. Vis. Exp. (54), e3324, doi:10.3791/3324 (2011).

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