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Isolement des ARN Pseudomonas aeruginosa La colonisation du tractus gastro-intestinal murin
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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Isolement des ARN Pseudomonas aeruginosa La colonisation du tractus gastro-intestinal murin

18,777 Views

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08:52 min

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September 28, 2011

DOI:

10.3791/3293-v

DOI:
10.3791/3293-v

•

08:52 min
September 28, 2011

•

6 Views
, , ,
1Department of Pediatrics,University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pediatrics and Microbiology,University of Texas Southwestern Medical Center

Summary

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Une méthode fiable pour l'isolement des ARN<em> Pseudomonas aeruginosa</em> Récupéré cecums murin est décrite. L'ARN récupéré est en quantité suffisante et de qualité pour qPCR subséquentes, le profilage de transcription, l'ARN et des expériences Seq. Cette technique peut être adaptée à l'isolement d'ARN des autres microbes intestinaux.

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Lopez-Medina, E., Neubauer, M. M., Pier, G. B., Koh, A. Y. RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract. J. Vis. Exp. (55), e3293, doi:10.3791/3293 (2011).

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