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Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
 

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice

Article DOI: 10.3791/3124-v • 07:10 min • September 8th, 2011
September 8th, 2011 •
1, 2, 3
1Biology Department, Arcadia University, 2Shriners Hospitals Pediatric Research Center, Temple University School of Medicine, 3Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine

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We describe procedures for repeated administration of inhibitors of muscarinic signaling to the levator auris longus (LAL) muscle of young adult mice and for subsequent immunostaining of its neuromuscular junctions (NMJs) in wholemounts. The LAL muscle has unique advantages for revealing in vivo pharmacological effects on NMJs.

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Subcutaneous Administration Muscarinic Antagonists Triple-immunostaining Levator Auris Longus Muscle Mice Hind Limb Muscles In Vivo Pharmacological Studies NMJs Drug Penetration Subcutaneous Administration Intramuscular Administration Systemic Administration Mini-pumps Inflammatory Responses Direct Muscle Damage Hind Limb Muscle Analysis Serial Sectioning Extensive Immunostaining Mouse LAL Muscle Pinna Movement Facial Nerve Branch Stylomastoid Foramen
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