/
/
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
Capitoli
- 00:05Titolo
- 02:08Tosylactivated Bead Coating
- 04:25PBMC Isolation
- 05:41MACS Pre-sort of CD4 T Cells
- 08:05Fluorescent Activated Cell Sorting (FACS) Isolation
- 09:59Cell Culture Setup, Harvesting, and Counting
- 11:32Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells
- 13:34Conclusion
Tregs are potent suppressors of the immune system. There is a lack of unique surface markers to define them, hence, definitions of Tregs are primarily functional. Here we describe an optimized in vitro assay capable of identifying immune imbalance in subjects at risk to develop T1D.
Tags
Human In Vitro SuppressionScreening ToolRecognitionEarly StateImmune ImbalanceRegulatory T CellsTregsImmune ToleranceSelf-antigensInfectionSurface MarkerProliferationFunctionEffector T CellsIn Vitro AssaysCross-sectional StudiesAutoimmune DiseasesType 1 Diabetes-T1DLaboratoriesResponder T CellsStimulationTreg:responder RatiosAntigen-presenting Cells (APC)VariabilityComparison Between StudiesStandardized Suppression AssayHealthy SubjectsAutoimmune Destruction Of Pancreatic β-cells
