Rivista
/
Immunologia e infezioni
/
人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
Please note that all translations are automatically generated. Click here for the English version.

人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具

DOI:
10.3791/3071-v

•

14:01 min

•

July 22, 2011

•

, ,
1Department of Pediatrics/Allergy,Medical College of Wisconsin , 2Flow Cytometry Core Facility,Medical College of Wisconsin , 3Max McGee National Research Center for Juvenile Diabetes and Human Molecular Genetics Center,Medical College of Wisconsin

Capitoli

  • 00:05Titolo
  • 02:08Tosylactivated Bead Coating
  • 04:25PBMC Isolation
  • 05:41MACS Pre-sort of CD4 T Cells
  • 08:05Fluorescent Activated Cell Sorting (FACS) Isolation
  • 09:59Cell Culture Setup, Harvesting, and Counting
  • 11:32Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells
  • 13:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

调节性T细胞是免疫系统的强有力的抑制器。有一个独特的表面标志物来定义它们的缺乏，因此，调节性T细胞的定义主要功能。在这里，我们描述了一个优化的<em>在体外</em>检测能够识别风险科目的免疫失衡发展T1D。

Tags

Human In Vitro SuppressionScreening ToolRecognitionEarly StateImmune ImbalanceRegulatory T CellsTregsImmune ToleranceSelf-antigensInfectionSurface MarkerProliferationFunctionEffector T CellsIn Vitro AssaysCross-sectional StudiesAutoimmune DiseasesType 1 Diabetes-T1DLaboratoriesResponder T CellsStimulationTreg:responder RatiosAntigen-presenting Cells (APC)VariabilityComparison Between StudiesStandardized Suppression AssayHealthy SubjectsAutoimmune Destruction Of Pancreatic β-cells

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

在细菌粘附的研究剪应力

寄生蜂简介<em>果蝇</em>和Antiparasite的免疫反应的

呼吸爆发的响应为先天免疫健康的斑马鱼中的指标量化

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

使用一个调查益生素对肺炎球菌定植的影响<em>体外</em>粘附试验

在 MyD88的介导的细胞免疫应答西尼罗河病毒变异株感染体外分析

一个<em>体外</em>模型在HIV合并感染的背景下测量免疫应答疟疾

高通量设置的建立筛选小分子能调节C-二GMP信令<em>绿脓杆菌</em

确定免疫系统抑制中枢神经系统对保护药物干预自身免疫性脱髓鞘

分析18葡萄糖 PET/CT 成像作为研究结核分枝杆菌感染和治疗非人类灵长类动物的工具

Read Article