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鼠标涎腺干细胞的分离
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Isolation of Mouse Salivary Gland Stem Cells
DOI:

09:52 min

February 08, 2011

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Capitoli

  • 00:05Titolo
  • 01:32Mechanical and Enzymatic Salivary Gland Tissue Digestion
  • 05:00Preparation of Salivary Gland Tissue for Plating
  • 07:32Plating Salivary Gland Cells
  • 08:39Examination of Salispheres and Proliferating Cells Using Phase-Contrast Microscopy and Immunostaining
  • 09:34Conclusion

Summary

Traduzione automatica

分离干细胞从小鼠唾液腺的优化协议描述。该方法采用酶法和机械消化,并允许包含干细胞的特征细胞salispheres的隔离。

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